Optogenetic regulation of transcription

Authors

Oksana Polesskaya, University of California, San Diego, CA, USA.
Ancha Baranova, Research Center for Medical Genetics RAMS, Moscow, Russia.
Sarah Bui, Center for the Study of Chronic Metabolic and Rare Diseases, School of Systems Biology, George Mason University, Fairfax, VA, USA.
Nikolai Kondratev, Localized Therapeutics, LLC, San Diego, CA, USA.
Evgeniya Kananykhina, Localized Therapeutics, LLC, San Diego, CA, USA.
Olga Nazarenko, Localized Therapeutics, LLC, San Diego, CA, USA.
Tatyana Shapiro, Localized Therapeutics, LLC, San Diego, CA, USA.
Frances Barg Nardia, Localized Therapeutics, LLC, San Diego, CA, USA.
Vladimir Kornienko, Localized Therapeutics, LLC, San Diego, CA, USA.
Vikas Chandhoke, Center for the Study of Chronic Metabolic and Rare Diseases, School of Systems Biology, George Mason University, Fairfax, VA, USA.
Istvan Stadler, Rochester Regional HealthFollow
Raymond Lanzafame, Raymond J. Lanzafame, MD PLLC, Rochester, NY, USA.
Max Myakishev-Rempel, Localized Therapeutics, LLC, San Diego, CA, USA.

Department

Surgery

Document Type

Article

Publication Title

BMC Neuroscience

Conference Name

Belyaev Conference 2017: neuroscience

Conference Date

2017

Abstract

Optogenetics has become widely recognized for its success in real-time control of brain neurons by utilizing non-mammalian photosensitive proteins to open or close membrane channels. Here we review a less well known type of optogenetic constructs that employs photosensitive proteins to transduce the signal to regulate gene transcription, and its possible use in medicine. One of the problems with existing gene therapies is that they could remain active indefinitely while not allowing regulated transgene production on demand. Optogenetic regulation of transcription (ORT) could potentially be used to regulate the production of a biological drug in situ, by repeatedly applying light to the tissue, and inducing expression of therapeutic transgenes when needed. Red and near infrared wavelengths, which are capable of penetration into tissues, have potential for therapeutic applications. Existing ORT systems are reviewed herein with these considerations in mind.

First Page

12

DOI

10.1186/s12868-018-0411-6

Volume

19

Issue

Suppl 1

Publication Date

4-19-2018

Medical Subject Headings

Animals; Brain (metabolism); Genetic Therapy; Humans; Light; Neurons (metabolism); Optogenetics (methods); Signal Transduction; Transcriptional Activation; Translational Research, Biomedical

PubMed ID

29745855

Recommended Citation

Polesskaya, O., Baranova, A., Bui, S., Kondratev, N., Kananykhina, E., Nazarenko, O., Shapiro, T., Nardia, F. B., Kornienko, V., Chandhoke, V., Stadler, I., Lanzafame, R., & Myakishev-Rempel, M. (2018). Optogenetic regulation of transcription. BMC Neuroscience, 19 (Suppl 1), 12. https://doi.org/10.1186/s12868-018-0411-6