Serum antibody response to Moraxella catarrhalis proteins in stringently defined otitis prone children

Department

Research

Document Type

Article

Publication Title

Vaccine

Abstract

BACKGROUND: Moraxella catarrhalis (Mcat) is a frequent pathogen of acute otitis media (AOM) in young children. Here we prospectively assessed naturally-induced serum antibodies to four Mcat vaccine candidate proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children age 6-36months old following nasopharyngeal (NP) colonization, at onset of AOM and convalescence from AOM. METHODS: Serum IgG and IgM antibody against recombinant Mcat proteins, oligopeptide permease A (OppA), outer membrane protein (OMP) CD, hemagglutinin (Hag), and PilA clade 2 (PilA2), were quantitated by ELISA. RESULTS: During NP colonization by Mcat all four antigens were immunogenic in both sOP and NOP children. However, sOP children had lower antibody responses than NOP children across age 6-36months, similar to our findings for protein vaccine candidates of Streptococcus pneumoniae (Spn) and Nontypeable Haemophilus influenzae (NTHi). sOP children displayed a later and lower peak of antibody rise than NOP children for all four antigens during NP colonization of Mcat. The age-dependent increase of antibody ranked as OppA>Hag5-9>OMP CD>PilA2 in both sOP and NOP children. Lower serum antibody levels to the Mcat antigens were measured in sOP compared to NOP children at the onset of AOM. We did not find a consistent significant increase of antibody at the convalescence phase after an AOM event. CONCLUSIONS: sOP children is a highly vulnerable population that mount lower serum antibody responses to Mcat candidate vaccine proteins compared to NOP children during asymptomatic NP carriage and at onset of AOM.

First Page

4637

Last Page

4645

DOI

10.1016/j.vaccine.2017.07.027

Volume

37

Issue

32

Publication Date

7-26-2019

Medical Subject Headings

Antibodies, Bacterial (blood); Antibody Formation (immunology); Antigens, Bacterial (immunology); Bacterial Outer Membrane Proteins (immunology); Bacterial Proteins (immunology); Child, Preschool; Female; Haemophilus Infections (blood, immunology); Haemophilus influenzae (immunology); Humans; Immunoglobulin G (immunology); Immunoglobulin M (immunology); Infant; Male; Membrane Transport Proteins (immunology); Moraxella catarrhalis (immunology); Nasopharynx (immunology); Otitis (blood, immunology); Otitis Media (immunology); Pneumococcal Infections (blood, immunology); Prospective Studies; Serum (immunology); Streptococcus pneumoniae (immunology)

PubMed ID

28755833

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