Title

Ampicillin triggers the release of Pal in toxic vesicles from Escherichia coli

Department

Research

Document Type

Article

Publication Title

International Journal of Antimicrobial Agents

Abstract

In addition to lipopolysaccharides (LPS), outer membrane proteins – Lpp, OmpA and peptidoglycan-associated lipoprotein (Pal) – are part of the outer membrane of Escherichia coli and are proposed to contribute to bacterial sepsis-related inflammation. This study showed that ampicillin (a β-lactam antibiotic) enhances Pal's release from Escherichia coli to a greater extent than gentamicin and levofloxacin (aminoglycoside and quinolone antibiotics, respectively). It is proposed that the majority of Pal is released in outer membrane vesicles (OMVs), which also contain LPS and other outer membrane and periplasmic proteins. The OMVs were purified by ultracentrifugation and characterised by transmission electron microscopy and nanoparticle tracking analysis, and Pal and other E. coli proteins were detected by Western blot. It also proposed that sepsis treatments using certain β-lactam antibiotics may further aggravate the over-exuberant inflammatory response by enhancing the release of Pal and LPS in OMVs.

DOI

10.1016/j.ijantimicag.2020.106163

Publication Date

12-1-2020

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