Ampicillin triggers the release of Pal in toxic vesicles from Escherichia coli
Department
Research
Document Type
Article
Publication Title
International Journal of Antimicrobial Agents
Abstract
In addition to lipopolysaccharides (LPS), outer membrane proteins – Lpp, OmpA and peptidoglycan-associated lipoprotein (Pal) – are part of the outer membrane of Escherichia coli and are proposed to contribute to bacterial sepsis-related inflammation. This study showed that ampicillin (a β-lactam antibiotic) enhances Pal's release from Escherichia coli to a greater extent than gentamicin and levofloxacin (aminoglycoside and quinolone antibiotics, respectively). It is proposed that the majority of Pal is released in outer membrane vesicles (OMVs), which also contain LPS and other outer membrane and periplasmic proteins. The OMVs were purified by ultracentrifugation and characterised by transmission electron microscopy and nanoparticle tracking analysis, and Pal and other E. coli proteins were detected by Western blot. It also proposed that sepsis treatments using certain β-lactam antibiotics may further aggravate the over-exuberant inflammatory response by enhancing the release of Pal and LPS in OMVs.
DOI
10.1016/j.ijantimicag.2020.106163
Volume
56
Issue
6
Publication Date
12-1-2020
Recommended Citation
Michel, L., Gallardo, L., Konovalova, A., Bauer, M., Jackson, N., Zavorin, M., McNamara, C., Pierce, J., Cheng, S., Snyder, E., Hellman, J., & Pichichero, M. E. (2020). Ampicillin triggers the release of Pal in toxic vesicles from Escherichia coli. International Journal of Antimicrobial Agents, 56 (6) https://doi.org/10.1016/j.ijantimicag.2020.106163