A panel of diverse Klebsiella pneumoniae clinical isolates for research and development

Authors

Melissa J. Martin, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
William Stribling, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Ana C. Ong, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Rosslyn Maybank, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Yoon I. Kwak, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Joshua A. Rosado-Mendez, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Lan N. Preston, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Katharine F. Lane, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Michael Julius, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Anthony R. Jones, Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Mary Hinkle, Rochester Regional HealthFollow
Paige E. Waterman, Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
Emil P. Lesho, Rochester Regional HealthFollow
Francois Lebreton, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Jason W. Bennett, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
Patrick T. Mc Gann, Multidrug-Resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.

Department

Infectious Diseases

Document Type

Article

Publication Title

Microbial Genomics

Abstract

Klebsiella pneumoniae are a leading cause of healthcare-associated infections worldwide. In particular, strains expressing extended-spectrum β-lactamases (ESBLs) and carbapenemases pose serious treatment challenges, leading the World Health Organization (WHO) to designate ESBL and carbapenem-resistant Enterobacteriaceae as 'critical' threats to human health. Research efforts to combat these pathogens can be supported by accessibility to diverse and clinically relevant isolates for testing novel therapeutics. Here, we describe a panel of 100 diverse K. pneumoniae isolates that are publicly available to assist the research community in this endeavour. Whole-genome sequencing (WGS) was performed on 3878 K. pneumoniae clinical isolates housed at the Multidrug-Resistant Organism Repository and Surveillance Network. The isolates were cultured from 63 facilities in 19 countries between 2001 and 2020. Core-genome multilocus sequence typing and high-resolution single-nucleotide polymorphism-based phylogenetic analyses captured the genetic diversity of the collection and were used to select the final panel of 100 isolates. In addition to known multidrug-resistant (MDR) pandemic lineages, the final panel includes hypervirulent lineages and isolates with specific and diverse resistance genes and virulence biomarkers. A broad range of antibiotic susceptibilities, ranging from pan-sensitive to extensively drug-resistant isolates, are described. The panel collection, and all associated metadata and genome sequences, are available at no additional cost and will be an important resource for the research community and for the design and development of novel antimicrobial agents and diagnostics against this important pathogen.

DOI

10.1099/mgen.0.000967

Volume

9

Issue

5

Publication Date

5-1-2023

Medical Subject Headings

Humans; Klebsiella pneumoniae; Anti-Bacterial Agents (pharmacology, therapeutic use); Phylogeny; Drug Resistance, Multiple, Bacterial (genetics); Research

PubMed ID

37141116

Recommended Citation

Martin, M. J., Stribling, W., Ong, A. C., Maybank, R., Kwak, Y. I., Rosado-Mendez, J. A., Preston, L. N., Lane, K. F., Julius, M., Jones, A. R., Hinkle, M., Waterman, P. E., Lesho, E. P., Lebreton, F., Bennett, J. W., & Mc Gann, P. T. (2023). A panel of diverse Klebsiella pneumoniae clinical isolates for research and development. Microbial Genomics, 9 (5) https://doi.org/10.1099/mgen.0.000967