Structure and IgE Cross-Reactivity among Cashew, Pistachio, Walnut, and Peanut Vicilin-Buried Peptides

Authors

• Alexander C. Foo, Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, 111 T.W. Alexander Drive, MD-MR01, Durham, North Carolina 27709, United States.
• Jacqueline B. Nesbit, Agricultural Research Service, United States Department of Agriculture, 1100 Allen Toussaint Boulevard, New Orleans, Louisiana 70124, United States.
• Stephen A. Gipson, Agricultural Research Service, United States Department of Agriculture, 1100 Allen Toussaint Boulevard, New Orleans, Louisiana 70124, United States.
• Eugene F. DeRose, Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, 111 T.W. Alexander Drive, MD-MR01, Durham, North Carolina 27709, United States.
• Hsiaopo Cheng, Agricultural Research Service, United States Department of Agriculture, 1100 Allen Toussaint Boulevard, New Orleans, Louisiana 70124, United States.
• Barry K. Hurlburt, Agricultural Research Service, United States Department of Agriculture, 1100 Allen Toussaint Boulevard, New Orleans, Louisiana 70124, United States.
• Michael D. Kulis, Department of Pediatrics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599-7220, United States.
• Edwin H. Kim, Department of Pediatrics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599-7220, United States.
• Stephen C. Dreskin, Division of Allergy and Clinical Immunology, University of Colorado Denver School of Medicine, Aurora, Colorado 80045-2560, United States.
• S. Shahzad Mustafa, Rochester Regional HealthFollow
• Soheila J. Maleki, Agricultural Research Service, United States Department of Agriculture, 1100 Allen Toussaint Boulevard, New Orleans, Louisiana 70124, United States.
• Geoffrey A. Mueller, Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, 111 T.W. Alexander Drive, MD-MR01, Durham, North Carolina 27709, United States.

Department

Allergy and Immunology

Document Type

Article

Publication Title

Journal of Agricultural and Food Chemistry

Abstract

Peanut and tree-nut allergies are frequently comorbid for reasons not completely understood. Vicilin-buried peptides (VBPs) are an emerging family of food allergens whose conserved structural fold could mediate peanut/tree-nut co-allergy. Peptide microarrays were used to identify immunoglobulin E (IgE) epitopes from the N-terminus of the vicilin allergens Ara h 1, Ana o 1, Jug r 2, and Pis v 3 using serum from three patient diagnosis groups: monoallergic to either peanuts or cashew/pistachio, or dual allergic. IgE binding peptides were highly prevalent in the VBP domains AH1.1, AO1.1, JR2.1, and PV3.1, but not in AO1.2, JR2.2, JR2.3, and PV3.2 nor the unstructured regions. The IgE profiles did not correlate with diagnosis group. The structure of the VBPs from cashew and pistachio was solved using solution-NMR. Comparisons of structural features suggest that the VBP scaffold from peanuts and tree-nuts can support cross-reactivity. This may help understand comorbidity and cross-reactivity despite a distant evolutionary origin.

First Page

2990

Last Page

2998

DOI

10.1021/acs.jafc.2c07061

Volume

71

Issue

6

Publication Date

2-15-2023

Medical Subject Headings

Humans; Nuts (metabolism); Juglans (metabolism); Anacardium (metabolism); Pistacia (metabolism); Arachis; Immunoglobulin E (metabolism); Nut Hypersensitivity (diagnosis); Allergens (chemistry); Peptides

PubMed ID

36728846

Recommended Citation

Foo, A. C., Nesbit, J. B., Gipson, S. A., DeRose, E. F., Cheng, H., Hurlburt, B. K., Kulis, M. D., Kim, E. H., Dreskin, S. C., Mustafa, S. S., Maleki, S. J., & Mueller, G. A. (2023). Structure and IgE Cross-Reactivity among Cashew, Pistachio, Walnut, and Peanut Vicilin-Buried Peptides. Journal of Agricultural and Food Chemistry, 71 (6), 2990-2998. https://doi.org/10.1021/acs.jafc.2c07061