Peripheral blood antigen presenting cell responses in otitis-prone and non-otitis-prone infants

Department

Research

Document Type

Article

Publication Title

Innate Immunity

Abstract

Stringently defined otitis-prone (sOP) children represent a new classification of the otitis-prone condition. Previous studies showed dysfunction in Ab, B-cell memory and T-cell memory responses. We sought to determine whether there are defects in numbers, phenotype and/or function of professional APC in the peripheral blood of sOP infants. APC phenotypic counts, MHC II expression and intracellular cytokine levels were determined in response to TLR7/8 (R848) stimulation by flow cytometry. Innate immune mRNA expression was measured using RT-PCR and cytokines were measured using Luminex technology. Significant (P < 0.05) increases in the phenotypic counts of monocytes and conventional dendritic cells but not plasmacytoid DCs were observed in sOP compared with non-otitis-prone (NOP) age-matched infants. No significant differences in APC activation or function were observed. Expression of various TLRs, intracellular signaling molecules and downstream cytokines was also not found to be significantly different between sOP and NOP infants. Higher numbers of APCs in sOP infants suggest the possibility of a persistent mucosal inflammatory status. Transcriptional and cytokine profiles of PBMCs among sOP infants suggest their systemic innate responses are not different compared to NOP infants.

First Page

63

Last Page

71

DOI

10.1177/1753425915616267

Volume

22

Issue

1

Publication Date

1-1-2016

Medical Subject Headings

Antigen-Presenting Cells (drug effects, immunology); Blood Cells (drug effects, immunology); Child, Preschool; Disease Susceptibility; Female; Humans; Imidazoles (pharmacology); Immunity, Innate; Immunophenotyping; Infant; Male; Otitis (diagnosis, immunology); Prospective Studies; Toll-Like Receptor 7 (agonists); Toll-Like Receptor 8 (agonists)

PubMed ID

26566651

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