Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus
Department
Medicine
Document Type
Article
Publication Title
PloS One
Abstract
Sensitive and precise serology assays are needed to measure the humoral response to antigens of respiratory syncytial virus (RSV) following natural infection or vaccination. We developed and evaluated a collection of electrochemiluminescent (ECL) serology assays using four RSV antigens (F, N, Ga and Gb). To assess the merits of ECL technology, the four ECL serology assays were evaluated using a well-characterized "gold standard" panel of acute and convalescent serum samples from fifty-nine RSV-positive and thirty RSV-negative elderly subjects (≥65 years old). The combined results from the four ECL assays demonstrated good concordance to the "gold standard" diagnosis, reaching 95% diagnostic sensitivity and 100% diagnostic specificity. Additionally, a combination of ECL assays provided higher diagnostic sensitivity than a commercially available diagnostic ELISA or cell-based microneutralization assay. In summary, these data demonstrate the advantages of using ECL-based serology assays and highlight their use as a sensitive diagnostic approach to detect recent RSV infection in an elderly population.
First Page
e0153019
DOI
10.1371/journal.pone.0153019
Volume
11
Issue
4
Publication Date
4-12-2016
Medical Subject Headings
Aged; Antibodies, Monoclonal, Humanized (immunology); Antibodies, Neutralizing (blood); Antibodies, Viral (blood); Antigens, Viral (immunology); Child, Preschool; Enzyme-Linked Immunosorbent Assay; Humans; Immunity, Humoral; Infant; Luminescent Measurements (methods); Neutralization Tests; Nucleocapsid Proteins (immunology); Respiratory Syncytial Virus Infections (diagnosis, immunology); Respiratory Syncytial Virus, Human (immunology); Serologic Tests (methods); Viral Envelope Proteins (immunology); Viral Fusion Proteins (immunology)
PubMed ID
27070145
Recommended Citation
Maifeld, S. V., Ro, B., Mok, H., Chu, M., Yu, L., Yamagata, R., Leonardson, T., Chio, V., Parhy, B., Park, S., Carlson, M., Machhi, S., Ulbrandt, N., Falsey, A. R., Walsh, E. E., Wang, C. K., Esser, M. T., & Zuo, F. (2016). Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus. PloS One, 11 (4), e0153019. https://doi.org/10.1371/journal.pone.0153019