Differences in innate immune response gene regulation in the middle ear of children who are otitis prone and in those not otitis prone

Department

Research

Document Type

Article

Publication Title

American Journal of Rhinology & Allergy

Abstract

OBJECTIVE: Acute otitis media (AOM) causes an inflammatory response in the middle ear. We assessed differences in innate immune responses involved in bacterial defense at onset of AOM in children who were stringently defined as otitis prone (sOP) and children not otitis prone (NOP). STUDY DESIGN: Innate immune genes analysis from middle ear fluid (MEF) samples of children. METHODS: Genes of toll-like receptors (TLR), nod-like and retinoic acid-inducible gene-I-like receptors, downstream effectors important for inflammation and apoptosis, including cytokines and chemokines, were studied from MEF samples by using a real-time polymerase chain reaction array. Protein levels of differentially regulated genes were measured by Luminex. RESULTS: Gene expression in MEF among children who were sOP was significantly different in upregulation of interleukin 8, secretory leukocyte peptidase inhibitor, and chemokine (C-C motif) ligand 3, and in downregulation of interferon regulatory factor 7 and its related signaling molecules interferon alpha, Toll-like receptor adaptor molecule 2, chemokine (C-C motif) ligand 5, and mitogen-activated protein kinase 8 compared with children who were NOP. Differences in innate gene regulation were similar when AOM was caused by Streptococcus pneumoniae or nontypeable Haemophilus influenzae. CONCLUSION: Innate-immune response genes are differentially regulated in children who were sOP compared with children with NOP.

First Page

218

Last Page

223

DOI

10.2500/ajra.2016.30.4393

Volume

30

Issue

6

Publication Date

11-1-2016

Medical Subject Headings

Adolescent; Chemokine CCL3 (genetics, metabolism); Chemokine CCL5 (genetics, metabolism); Child; Ear, Middle (metabolism, pathology); Female; Gene Expression Regulation (immunology); Genetic Predisposition to Disease; Haemophilus parainfluenzae; Humans; Immunity, Innate (genetics); Interferon Regulatory Factor-7 (genetics, metabolism); Interferon-alpha (genetics, metabolism); Interleukin-8 (genetics, metabolism); Male; Otitis Media (genetics, immunology); Prospective Studies; Secretory Leukocyte Peptidase Inhibitor (genetics, metabolism); Streptococcus pneumoniae; Toll-Like Receptor 2 (genetics, metabolism)

PubMed ID

28124644

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